Composite

Part:BBa_K209431:Design

Designed by: Katja Kolar   Group: iGEM09_UCSF   (2009-10-02)

SSFYFP-hM2D-Beta Pix


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 721
    Illegal XbaI site found at 5326
    Illegal XbaI site found at 6748
    Illegal PstI site found at 2984
    Illegal PstI site found at 5481
    Illegal PstI site found at 5747
    Illegal PstI site found at 6068
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 239
    Illegal NheI site found at 505
    Illegal NheI site found at 3799
    Illegal PstI site found at 2984
    Illegal PstI site found at 5481
    Illegal PstI site found at 5747
    Illegal PstI site found at 6068
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2766
    Illegal BamHI site found at 706
    Illegal BamHI site found at 3593
    Illegal BamHI site found at 4303
    Illegal XhoI site found at 5630
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 721
    Illegal XbaI site found at 5326
    Illegal XbaI site found at 6748
    Illegal PstI site found at 2984
    Illegal PstI site found at 5481
    Illegal PstI site found at 5747
    Illegal PstI site found at 6068
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 721
    Illegal XbaI site found at 5326
    Illegal XbaI site found at 6748
    Illegal PstI site found at 2984
    Illegal PstI site found at 5481
    Illegal PstI site found at 5747
    Illegal PstI site found at 6068
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2722
    Illegal BsaI.rc site found at 5531
    Illegal BsaI.rc site found at 6833
    Illegal SapI.rc site found at 116
    Illegal SapI.rc site found at 5137


Design Notes

Cloned into BBa_K209399.

Source

References